Due to its high vitamin content Brucella, Pasteurella and Streptococcus are perfectly viable, moreover a CO2 enriched atmosphere can further fenhance growth. In anaerobic conditions this broth will grow Bacteroides and Clostridium species. For this purpose, the best results can be obtained by adding 0.3% agar and 0.05% sodium azide for Clostridium. Tryptic Soy Broth’s superior growth-promoting properties make it particularly suitable for tube dilution methods for antibiotic sensitivity testing.