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SCHARLAU Mannitol Salt Agar

01-116-500

Mannitol Salt Agar is a classical medium for the detection and enumeration of staphylococci. It was described by Chapman and has been adopted by many official organisations. Several modifications of it have been developed, all formulations resulting in media with similar efficiency.

This medium takes advantage of the high saline tolerance of staphylococci, and uses sodium chloride as a selective agent. Only staphylococci and halophilic enterobacteria are able to grow freely at the concentration of salt employed in this medium, while other bacteria are inhibited. It also exploits the correlation between the pathogenicity of staphylococci and their ability ferment mannitol. Mannitol fermentation results in an accumulation of acid products, indicated by the phenol red indicator turning yellow. A yellow halo surrounds the presumptive pathogenic colonies, while the rest of the medium remains red/orange in colour