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Hemacolor® Rapid staining – Solution 3

Hemacolor® Rapid staining of blood smear – Solution 3: colour reagent blue, is a ready-to-use solution used for human-medical cell diagnosis and serves the fixing of native blood and bone-marrow smears of human origin in hematological and clinico-cytological specimen materials, for example smears of whole blood and bone marrow. The staining with Hemacolor® yields a staining result that corresponds to the Pappenheim stain, with predominantly magenta stained nuclei.

This is based on the molecular interaction of the Eosin Y dye and a complex of Azur B with DNA. Both dyes assemble to an Eosin Y - Azur B-DNA complex and the intensity of the resulting stain depends on the content of Azur B and the ratio of Azur B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. By using pH 7.2 buffered solutions with the Hemacolor® Rapid staining of blood smear solutions, a high stability of the stain and clean, precipitation-free staining results can be guaranteed.
The package is sufficient for 1250 - 5000 applications. The buffer tablets acc. to Weise pH 7.2 (Product number 1.09468) are also available in larger pack sizes, thus, they can be useful for higher demands.